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MOLECULAR BIOLOGY: WORKING WITH PROTEINS

EXPRESSION: FUSION PROTEINS

1. Expression and Detection of Transiently Transfected Flag-tagged Receptor in Tissue Culture Cells
2. GST Pull-Downs from Drosophila Tissue Culture Cells
3. GTP Blots for the Identification of GTP-Binding Proteins
4. Induction of Bacterial Expression of Heterologous Motor Proteins
5. Native Purification of His-Tagged Proteins From Sf9 Cells Using Ni-NTA
6. Preparation and Use of a Glutathione S-Transferase Column - A General Method
7. Protein Purification of Py-Tagged Proteins from Baculovirus-Infected Insect Cells Using Anti-Py-Protein-G-Sepharose
8. Protein Purification of Py-Tagged Proteins from Vaccinia Virus-Infected Cells Using Anti-Py-Protein-G-Sepharose
9. Purification of Bacterially Expressed Proteins from Inclusion Bodies
10. Purification of Bacterially Produced His-tagged Proteins on a Nickel Column
11. Purification of Expressed Proteins from Baculovirus-Infected Sf9 Insect Cells Using Epitope-Tag Affinity Purification
12. Purification of Expressed Proteins from Baculovirus-Infected Sf9 Insect Cells Using Metal Chelation Chromatography
13. Purification of GST-Fusion Proteins from E. coli
14. Purification of GST-Fusion Proteins from E. coli-Alternate Protocol
15. Purification of GST-Fusion Proteins from E. coli-Alternate Protocol 2
16. Purification of GST-Fusion Proteins from E. coli-Basic Protocol
17. Purification of Insoluble Over-Expressed Proteins Under the Control of the T7 Promoter in E. coli
18. Purification of Maltose Binding Protein-Fusion Proteins from E. coli
19. Western Blot Detection



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